Walker’s Toadfish was described in 1981; it is known only from the holotype that was found 1953 in the Panama Bay, Panama.
The type locality is considered to be in an eutrophic state due to ongoing pollution from agricultural- and livestock operations as well as from urban wastewaters from Panama City; Walker’s Toadfish might well be extinct now.
Rabb’s Fringe-limbed Treefrog, described in 2008, was only ever known from a single locality in Panama.
The species reached sizes of up to 10 cm.
The wild population of this species collapsed shortly after its discovery in 2005 due to chytridiomycosis, which killed countless amphibian populations across Central America.
The species was considered functionally extinct since the 2010s because at that time there was apparently only one single specimen left, a male that was kept in the Botanical Garden of the city of Atlanta in Georgia, USA.
This last known member of its species (see photo), however, died today, September 26, 2016, thus this species is now indeed fully extinct.
The Chiriqui Stub-foot Toad was described in 1936, it was restricted to a small range in the vicinity of the Chiriquí Viejo River in the Chiriquí Province, Panama.
The species fell victim to the spread of the fungal chytridiomycosis disease, which is deadly to amphibians, in the 2000s, it was not recorded in field surveys undertaken in 2012 and is now feared to be already extinct. 
 Rachel Perez; Corinne L. Richards-Zawacki; Alexander R. Krohn; Matthew Robak; Edgardo J. Griffith; Heidi Ross; Brian Gratwicke; Roberto Ibáñez; Jamie Voyles: Field surveys in western Panama indicated populations of Atelopus varius frogs are persisting in regions where Batrachochytrium dendrobatidis is now enzootic. Ambiphian & Reptile Conservation 8(2): 30-35. 2014